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r2a broth powder  (Nihon Pharmaceutical CO)

 
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    Nihon Pharmaceutical CO r2a broth powder
    R2a Broth Powder, supplied by Nihon Pharmaceutical CO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/r2a+broth+powder/r2a+broth/pm28875922-41-30-28
    Average 90 stars, based on 1 article reviews
    r2a broth powder - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Marinibactrum halimedae gen. nov., sp. nov., a gammaproteobacterium isolated from a marine macroalga.
    Article Snippet: Cultivation of the isolated bacterium was conducted at 25 8C using 1/10 MB agar and R2A-ASW agar, which was composed of (per litre ASW) 3.2 g R2A broth (Nihon Pharmaceutical) powder and 15 g agar, pH 7.2.



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    r2a broth powder  (Neogen)
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    Neogen r2a broth powder
    A | After pre-culturing each of the 16 species of the community for one growth cycle, we adjusted optical density of each culture, pooled them at equal volume and inoculated 96 resource environments, with each condition replicated 8 times. Each culture was serially transferred for 16 days, with 1% of the culture transferred every 48 h. B | At each transfer, we measured OD 600 of each community. In addition, we conducted 16S rRNA sequencing of all communities at the end-point and measured growth traits of each of the dominant species ( A. caviae, P. chlororaphis, C. koseri ) in monoculture in all carbon levels in the absence of <t>R2A.</t> Alt text. Two-panel schematic representing the study set-up. Panel A describes parameters of the serial passaging experiment. Panel B explains analytical procedures of the community and single-species experiments.
    R2a Broth Powder, supplied by Neogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/r2a+broth+powder/broth+medium+r2a/bio_rxiv__64898__2026__02__26__708221-48-6-9
    Average 86 stars, based on 1 article reviews
    r2a broth powder - by Bioz Stars, 2026-09
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    		  Buy from Supplier
    r2a broth powder  (Nihon Pharmaceutical CO)
    90
    Nihon Pharmaceutical CO r2a broth powder
    A | After pre-culturing each of the 16 species of the community for one growth cycle, we adjusted optical density of each culture, pooled them at equal volume and inoculated 96 resource environments, with each condition replicated 8 times. Each culture was serially transferred for 16 days, with 1% of the culture transferred every 48 h. B | At each transfer, we measured OD 600 of each community. In addition, we conducted 16S rRNA sequencing of all communities at the end-point and measured growth traits of each of the dominant species ( A. caviae, P. chlororaphis, C. koseri ) in monoculture in all carbon levels in the absence of <t>R2A.</t> Alt text. Two-panel schematic representing the study set-up. Panel A describes parameters of the serial passaging experiment. Panel B explains analytical procedures of the community and single-species experiments.
    R2a Broth Powder, supplied by Nihon Pharmaceutical CO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/r2a+broth+powder/r2a+broth/pm28875922-41-30-28
    Average 90 stars, based on 1 article reviews
    r2a broth powder - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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    A | After pre-culturing each of the 16 species of the community for one growth cycle, we adjusted optical density of each culture, pooled them at equal volume and inoculated 96 resource environments, with each condition replicated 8 times. Each culture was serially transferred for 16 days, with 1% of the culture transferred every 48 h. B | At each transfer, we measured OD 600 of each community. In addition, we conducted 16S rRNA sequencing of all communities at the end-point and measured growth traits of each of the dominant species ( A. caviae, P. chlororaphis, C. koseri ) in monoculture in all carbon levels in the absence of R2A. Alt text. Two-panel schematic representing the study set-up. Panel A describes parameters of the serial passaging experiment. Panel B explains analytical procedures of the community and single-species experiments.

    Journal: bioRxiv

    Article Title: Ecological tristability driven by total carbon availability over resource complexity in a synthetic microbial community

    doi: 10.64898/2026.02.26.708221

    Figure Lengend Snippet: A | After pre-culturing each of the 16 species of the community for one growth cycle, we adjusted optical density of each culture, pooled them at equal volume and inoculated 96 resource environments, with each condition replicated 8 times. Each culture was serially transferred for 16 days, with 1% of the culture transferred every 48 h. B | At each transfer, we measured OD 600 of each community. In addition, we conducted 16S rRNA sequencing of all communities at the end-point and measured growth traits of each of the dominant species ( A. caviae, P. chlororaphis, C. koseri ) in monoculture in all carbon levels in the absence of R2A. Alt text. Two-panel schematic representing the study set-up. Panel A describes parameters of the serial passaging experiment. Panel B explains analytical procedures of the community and single-species experiments.

    Article Snippet: R2A medium was prepared by dissolving R2A Broth powder (Neogen, UK) in M9 minimal medium.

    Techniques: Sequencing, Passaging

    A | Shannon diversity shown separately for each resource environment. The x- axis shows the total carbon level (doubling concentrations spaced evenly) and point color indicates resource complexity level. The figure contains 768 data points (8 replicates in 96 resources). B | Hump metric for total carbon gradient ( y -axis) at each level of resource complexity ( x -axis). Points show the estimated peak height (hump statistic); error bars show 95 % bootstrap confidence intervals. C | Variance partitioning of Shannon diversity using nested linear models. Bars show unique R 2 contributions of total carbon level, resource complexity (% R2A carbon contribution), and their interaction. Alt text. Three-panel figure representing diversity measures, with panel A containing data on Shannon diversity, resolved for each resource environment, panel showing a hump metric for total carbon gradient ( y -axis) at each level of resource complexity ( x -axis), and panel C showing variance partitioning of Shannon diversity using nested linear models.

    Journal: bioRxiv

    Article Title: Ecological tristability driven by total carbon availability over resource complexity in a synthetic microbial community

    doi: 10.64898/2026.02.26.708221

    Figure Lengend Snippet: A | Shannon diversity shown separately for each resource environment. The x- axis shows the total carbon level (doubling concentrations spaced evenly) and point color indicates resource complexity level. The figure contains 768 data points (8 replicates in 96 resources). B | Hump metric for total carbon gradient ( y -axis) at each level of resource complexity ( x -axis). Points show the estimated peak height (hump statistic); error bars show 95 % bootstrap confidence intervals. C | Variance partitioning of Shannon diversity using nested linear models. Bars show unique R 2 contributions of total carbon level, resource complexity (% R2A carbon contribution), and their interaction. Alt text. Three-panel figure representing diversity measures, with panel A containing data on Shannon diversity, resolved for each resource environment, panel showing a hump metric for total carbon gradient ( y -axis) at each level of resource complexity ( x -axis), and panel C showing variance partitioning of Shannon diversity using nested linear models.

    Article Snippet: R2A medium was prepared by dissolving R2A Broth powder (Neogen, UK) in M9 minimal medium.

    Techniques: